Coding

Part:BBa_K270005:Design

Designed by: Ann Lesnefsky   Group: iGEM09_Michigan   (2009-10-19)


XylR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 643
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 466
    Illegal PstI site found at 643
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 86
    Illegal BglII site found at 1548
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 643
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 643
    Illegal NgoMIV site found at 252
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 739


Design Notes

This part was obtained using colony PCR of the entire Pr XylR region on the pWW0 plasmid.


Source

NCBI: Pseudomonas putida plasmid pWW0

References